All Optical Electrophysiology
Dempsey, Graham T., et al. “Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging.” Journal of pharmacological and toxicological methods (2016).
Zhang, Hongkang, Elaine Reichert, and Adam E. Cohen. “Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.” eLife 5 (2016): e15202.
Abdelfattah, Ahmed S., et al. “A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices.” The Journal of Neuroscience 36.8 (2016): 2458-2472.
Brinks, Daan, Aaron J. Klein, and Adam E. Cohen. “Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage.” Biophysical journal 109.5 (2015): 914-921.
Shan Lou, Yoav Adam, Eli N. Weinstein, Erika Williams, Katherine Williams, Vicente Parot, Nikita Kavokine, Stephen Liberles, Linda Madisen, Hongkui Zeng, and Adam E. Cohen, “Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse”, The Journal of Neuroscience, 26 October 2016, 36(43):11059-11073; doi:10.1523/JNEUROSCI.1582-16.2016
J. H. Hou JH, J. M. Kralj, A. D. Douglass, F. Engert, A. E. Cohen, “Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents”, Front. Physiol., 2014 | doi: 10.3389/fphys.2014.00344
D. Hochbaum*, Y. Zhao*, S. Farhi, N. Klapoetke, C. A. Werley, V. Kapoor, P. Zou, J. M. Kralj, D. Maclaurin, N. Smedemark-Margulies, J. Saulnier, G. L. Boulting, C. Straub, Y.K. Cho, M. Melkonian, G. K-S. Wong, D. J. Harrison, V. Murthy, B. Sabatini, E. S. Boyden, R. E. Campbell, A. E. Cohen, “All optical electrophysiology in mammalian neurons using engineered microbial rhodopsins”, Nature Methods, 2014, doi:10.1038/nmeth.3000
P. Zou, Y. Zhao, A. D. Douglass, D. R. Hochbaum, D. Brinks, C. A. Werley, D. J. Harrison, R. E. Campbell, A. E. Cohen, “Bright and fast voltage reporters across the visible spectrum via electrochromic FRET (eFRET)”, arXiv.org (2014) http://arxiv.org/abs/1403.4636
V. Venkatachalam, D. Brinks, D. Maclaurin, D. Hochbaum, A. E. Cohen, “Flash Memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin,” J. Am. Chem. Soc. (2014) Vol. 136, pp. 2529-2537
J. Hou, V. Venkatachalam, A. E. Cohen, “Temporal dynamics of microbial rhodopsin fluorescence reports absolute membrane voltage,” Biophysical Journal, (2014) Vol. 106, pp. 639-648
J. Park*, C. A. Werley*, V. Venkatachalam, S. Dib-Hajj, S. Waxman, A. E. Cohen, “Screening fluorescent voltage indicators with spontaneously spiking HEK cells,” PLoS One, (2013) Vol. 8, pp. e85221
D. Maclaurin*, V. Venkatachalam*, H. Lee, A. E. Cohen (*Co-first authors), “Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin,” PNAS, (2013) Vol. 110, pp. 5939-5944
J. Kralj*, A. D. Douglass*, D. R. Hochbaum*, D. Maclaurin, A. E. Cohen (*Co-first authors), “Optical recording of action potentials in mammalian neurons using a microbial rhodopsin,” Nature Methods, (2012) Vol. 9, pp. 90-95
J. Kralj, D. R. Hochbaum, A. D. Douglass, A. E. Cohen, “Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein,” Science, (2011) Vol. 333, pp. 345-348
Stem Cell Biology
Burberry, Aaron, et al. “Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.” Science Translational Medicine 8.347 (2016): 347ra93-347ra93.
Sances, Samuel, et al. “Modeling ALS with motor neurons derived from human induced pluripotent stem cells.” Nature neuroscience 16.4 (2016): 542-553.
Sun, Shuying, et al. “ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP.” Nature communications 6 (2015).
Merkle, Florian T., et al. “Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.” Cell reports 11.6 (2015): 875-883.
Toma, Jeremy S., et al. “Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons.” The Journal of Neuroscience 35.3 (2015): 1291-1306.
Paull, Daniel, et al. “Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.” Nature methods 12.9 (2015): 885-892.
McNeish, John, et al. “From dish to bedside: Lessons learned while translating findings from a stem cell model of disease to a clinical trial.” Cell stem cell 17.1 (2015): 8-10.
Feng Tian, Wenlong Yang, Daniel A. Mordes, Jin-Yuan Wang, Johnny S. Salameh, Joanie Mok, Jeannie Chew, Aarti Sharma, Ester Leno-Duran, Satomi Suzuki-Uematsu, Naoki Suzuki, Steve S. Han, Fa-Ke Lu, Minbiao Ji, Rosanna Zhang, Yue Liu, Jack Strominger, Neil A. Shneider, Leonard Petrucelli, X. Sunney Xie, and Kevin EgganFeng Tian, “Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging”, Nat. Commun. 7, 13283 doi: 10.1038/ncomms13283 (2016).
J. S. Toma, B. C. Shettar, P. H. Chipman, D. M. Pinto, J. P. Borowska, J. K. Ichida, J. P. Fawcett, Y. Zhang, K. Eggan, V. F. Rafuse, “Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons”, J Neurosci. 2015 Jan 21;35(3):1291-306. doi: 10.1523/JNEUROSCI.2126-14.2015.
B. J. Wainger, E. D. Buttermore, J. T. Oliveira, C. Mellin, S. Lee, W. A. Saber, A. J. Wang, J. K. Ichida, I. M. Chiu, L. Barrett, E. A. Huebner, C. Bilgin, N. Tsujimoto, C. Brenneis, K. Kapur, L. L. Rubin, K. Eggan, C. J. Woolf, “Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts”, Nat Neurosci. 2015 Jan;18(1):17-24. doi: 10.1038/nn.3886. Epub 2014 Nov 24.
A. S. de Boer, K. Koszka, E. Kiskinis, N. Suzuki, B. N. Davis-Dusenbery and K. Eggan, “Genetic validation of a therapeutic target in a mouse model of ALS,” 6 August 2014, Vol. 6 no. 248 pp. 248ra104. DOI:10.1126/scitranslmed.3009351 248ra104
j. K. Ichida, J. TCW, L. A. Williams, A. C. Carter, Y. Shi, M. T. Moura, M. Ziller, S. Singh, G. Amabile, C. Bock, A. Umezawa, L. L. Rubin, J. E. Bradner, H. Akutsu, A. Meissner and K. Eggan, “Notch inhibition allows oncogene-independent generation of iPS cells,” Nat Chem Biol. 2014 Aug;10(8):632-9. doi: 10.1038/nchembio.1552. Epub 2014 Jun 22.
E. Kiskinis, J. Sandoe, L. A. Williams, G. L. Boulting, R. Moccia, B. J. Wainger, S. Han, T. Peng, S. Thams, S. Mikkilineni, C. Mellin, F. T. Merkle, B. N. Davis-Dusenbery, M. Ziller, D. Oakley, J. Ichida, S. Di Costanzo, N. Atwater, M. L. Maeder, M. J. Goodwin, J. Nemesh, R. E. Handsaker, D. Paull, S. Noggle, S. A. McCarroll, J. K. Joung, C. J. Woolf, R. H. Brown and K. Eggan, “Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1,” Cell Stem Cell, (2014) Vol. 14: Issue 6, pp. 781-795
B. J. Wainger, E. Kiskinis, C. Mellin, O. Wiskow, S. S. Han, J. Sandoe, N. P. Perez, L. A. Williams, S. Lee, G. Boulting, J. D. Berry, R. H. Jr Brown, M. E. Cudkowicz, B. P. Bean, K. Eggan and C. J. Woolf, “Intrinsic membrane hyperexcitability of amyotrophic lateral sclerosis patient-derived motor neurons,” Cell Reports, (2014) Vol. 7: Issue 1, pp. 1-11
C. S. Navara, J. Hornecker, D. Grow, S. Chaudhari, P. J. Hornsby, J. K. Ichida, K. Eggan and J. R. McCarrey, “Derivation of induced pluripotent stem cells from the baboon: a nonhuman primate model for preclinical testing of stem cell therapies,” Cellular Reprogramming, (2013) Vol. 15: Issue 6, pp. 495-502
E. Y. Son, J. K. Ichida, B. J. Wainger, J. S. Toma, V. F. Rafuse, C. J. Woolf and K. Eggan, “Conversion of mouse and human fibroblasts into functional spinal motor neurons,” Cell Stem Cell, (2011) Vol. 9: Issue 3, pp. 205-18
C. Bock, E. Kiskinis, G. Verstappen, H. Gu, G. Boulting, Z. D. Smith, M. Ziller, G. F. Croft, M. W. Amoroso, D. H. Oakley, A. Gnirke, K. Eggan and A. Meissner, “Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines,” Cell, (2011) Vol. 144: Issue 3, pp. 439-52
G. L. Boulting, E. Kiskinis, G. F. Croft, M. W. Amoroso, D. H. Oakley, B. J. Wainger, D. J. Williams, D. J. Kahler, M. Yamaki, L. Davidow, C. T. Rodolfa, J. T. Dimos, S. Mikkilineni, A. B. MacDermott, C. J. Woolf, C. E. Henderson, H. Wichterle and K. Eggan, “A functionally characterized test set of human induced pluripotent stem cells,” Nature Biotechnology, (2011) Vol. 3, pp. 279-86
F. P. Di Giorgio, G. L. Boulting, S. Bobrowicz and K. Eggan, “Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation,” Cell Stem Cell, (2008) Vol. 3: Issue 6, pp. 637-48
J. T. Dimos, K. T. Rodolfa, K. K. Niakan, L. M. Weisenthal, H. Mitsumoto, W. Chung, G. F. Croft, G. Saphier, R. Leibel, R. Goland, H. Wichterle, C. E. Henderson and K. Eggan, “Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons,” Science, (2008), Vol. 321: Issue 5893, pp. 1218-21