Q-State seeks to publish the learnings from our work so that this information is available to the scientific community and can be used to advance the study of genetic disorders.

McManus OB, Werley CA, Dempsey GT. Inhale, exhale: probing the inside-out mechanism of nicotine addiction using novel fluorescent sensors. J Gen Physiol 2019; J Gen Physiol 2019;151(6):714-717. PMID: 30902836

Nguyen C, Upadhyay H, Murphy M, et al. Simultaneous voltage and calcium imaging and optogenetic stimulation with high sensitivity and a wide field of view. Biomed Opt Express 2019;10(2):789-806. PMID 30800515

Williams LA, Joshi V, Murphy M, et al. Scalable measurements of intrinsic excitability in human iPS cell-derived excitatory neurons using all-optical electrophysiology. Neurochem Res 2019;44(3):714-725. PMID 30603979

Werley CA, Brookings T, Upadhyay H, et al. All-optical electrophysiology for disease modeling and pharmacological characterization of neurons. Curr Protoc Pharmacol 2017;78:11.21.1-11.20.24. PMID 28892145

Hempel CM, Werley CA, Dempsey GT, et al. Targeting neuronal function for CNS drug discovery. Drug Discov Today Technol 2017;23:17-25. PMID: 28647082

Dempsey GT, Chaudhary KW, Atwater N, et al. Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging. J Pharmacol Toxicol Methods 2016;81:240-250. PMID: 27184445


Q-State Co-Founders and Harvard Professors, Adam Cohen and Kevin Eggan, also regularly publish research in the areas of stem cell biology, genetics, and optical electrophysiology. Select publications include:

Adam Y, Kim JJ, Lou S…Cohen AE. Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. Nature 2019;569(7756):413-417. PMID: 31043747

Klim JR, Williams LA, Limone F…Eggan K. ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair. Nat Neuroscience 2019;22(2):167-179. PMID: 30643292

Fan LZ, Nehme R, Adam Y…Cohen AE. All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. Nat Methods 2018;15(10):823-831. PMID: 30275587

Shi Z, Graber ZT, Baumgart T…Cohen AE. Cell membranes resist flow. Cell 2018;175(7):1769-1779. PMID: 30392960

Cohen AE, Farhi SL. Sculpting light to reveal brain function. Nat Neurosci 2018;21(6):776-778. PMID: 29802389

Nehme R, Zuccaro E, Ghosh SD…Eggan K. Combining NGN2 programming with developmental patterning generates human excitatory neurons with NMDAR-mediated synaptic transmission. Cell Rep 2018;23(8):2509-2523. PMID: 29791859

Merkle FT, Ghosh S, Kamitaki N…Eggan K. Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations. Nature 2017;545(7653):229-233. PMID: 28445466

Tian F, Yang W, Mordes DA…Eggan K. Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging. Nat Commun 2016;7:13283. PMID: 27796305

Merkle FT, Neuhausser WM, Santos D…Eggan K. Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Cell Rep 2015;11(6):875-883. PMID: 25937281

De Boer AS, Koszka K, Kiskinis E…Eggan K. Genetic validation of a therapeutic target in a mouse model of ALS. Sci Transl Med 2014;6(248). PMID: 25100738

Kiskinis E, Sandoe J, Williams LA…Eggan K. Pathways disrupted in human ALS motor neurons identified through genetic correction of mutant SOD1. Cell Stem Cell 2014;14(6):781-795. PMID: 24704492

Zou P, Zhao Y, Douglass AD…Cohen AE. Bright and fast multicolored voltage reporters via electrochromic FRET. Nat Commun 2014:5(4625). PMID: 25118186

Hochbaum DR, Zhao Y, Farhi SL…Cohen AE. All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat Methods 2014;11(8):825-833. PMID: 24952910

Ichida JK, Tcw, J, Williams LA…Eggan K. Notch inhibition allows oncogene-independent generation of iPS cells. Nat Chem Biol 2014;10(8):632-639. PMID: 24952596

Suzuki N, Maroof AM, Merkle FT…Eggan K. The mouse C9ORF72 ortholog is enriched in neurons known to degenerate in ALS and FTD. Nat Neuroscience 2013;16(12):1725-1727. PMID: 24185425

Merkle FT, Eggan K. Modeling human disease with pluripotent stem cells: from genome association to function. Cell Stem Cell 2013;12(6):656-658. PMID: 23746975

Sandoe J, Eggan K. Opportunities and challenges of pluripotent stem cell neurodegenerative disease models. Nat Neurosci 2013;16(7):780-9. PMID: 23799470

Kralj JM, Douglass AD, Hochbaum DR…Cohen AE. Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Nat Methods 2011;9(1):90-95. PMID: 22120467

Kralj JM, Hochbaum DR, Douglass AD…Cohen AE. Electrical spiking in Echerichia coli probed with a fluorescent voltage-indicating protein. Science 2011;333(6040):245-248. PMID: 21764748

Tang Y, Cohen AE. Enhanced enatioselectivity in excitation of chiral molecules by superchiral light. Science 2011;332(6072):333-6. PMID: 21493854

Son EY, Ichida JK, Wainger BJ…Eggan K. Conversion of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell 2011;9(3):2015-2018. PMID: 21852222

Boulting GL, Kiskinis E, Croft GF…Eggan K. A functionally characterized test set of human induced pluripotent stem cells. Nat Biotechnol 2011;29(3):279-286. PMID: 21293464

Ichida JK, Blanchard J, Lam K…Eggan K. A small-molecule inhibitor of tgf-Beta signaling replaces sox2 in reprogramming by inducing nanog. Cell Stem Cell 2009;5(5):491-503. PMID: 19818703

Di Giorgio FP, Boulting GL, Bobrowicz S, Eggan KC. Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. Cell Stem Cell 2008;3(6):637-648. PMID 19041780

Egli D, Birkhoff G, Eggan K. Mediators of reprogramming: transcription factors and transitions through mitosis. Nat Rev Mol Cell Biol 2008;9(7):505-16. PMID: 18568039

Dimos JT, Rodolfa kT, Niakan KK, …. Eggan K. Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 2008;321(5893):1218-1221. PMID: 18669821